Results
The results of the first project year are now available!
In WP1, the following objectives have been achieved: (1) the construction of BAC libraries for chromosome 3B (version 2) and from chromosome arms 3DS, ADL, 1BS and 1AL, (2) the fingerprinting of the 3Bv2 and 3B MTP1 BAC clones to complete the physical map of chromosome 3B as well as fingerprinting of the 3DL and 3DS BAC libraries, (3) the assembly of the 3Bv2 and 3DL physical maps and the design of the corresponding MTP.
WP2 permitted to (1) establish a database with all potential markers for group 1 and group 3 chromosomes and (2) initiate the development of high-resolution mapping populations in wheat and barley for meiotic anchoring of the physical maps with these markers.
For the first project year, WP3 partners have focused on establishing the genetic and molecular resources needed for fine genetic mapping of the targeted genes and QTL through (1) the construction of high-resolution mapping populations and (2) the collection of molecular markers in the target regions and the evaluation of their polymorphism in the parents of the mapping populations.
In WP4, the following objectives have been achieved: (1) the development of BAC pools for the whole genome ‘Chinese Spring’ (CS) BAC library and the development of bioinformatics tools for the generation of gene-based markers from BAC end sequences and, (2) the assessment of general adaptation and genetic diversity of a primary diversity panel of 743 winter wheat varieties from UK, France and Germany and the identify a sub-set of 384 varieties for multi-location yield components trials in 2010.
During the first year, databases at INRA and HGMU were optimized to prepare their implementation with marker data that will be useful for the physical map anchoring and map-based cloning projects. Marker types and common exchange format were agreed. In addition, a new analytical framework for Linear Topology Contig (LTC) assembly has been developed and its elements have been tested on data from the wheat 3B physical map.
You can download a more complete file here
The Public Deliverables and Publications are available below:
State of progress of the tasks and deliverables per WPs:
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This project is supported by the European Commission under the 7th Framework Programme
for Research and Technological Development