WP 3 - Gene isolation and new allele discovery
WP Leader: Dr. Laura Rossini (UMIL, E-mail: laura.rossini@unimi.it)
WP3 aims to isolate five agronomically important genes and QTLs (Quantitative Trait Loci), 4 in wheat and 1 in barley. For each specific site of a particular gene, on its chromosome (locus), WP3 will integrate information and tools developed in WPs 1, 2, and 5 to achieve the following objectives:
- high resolution mapping of the target locus exploiting the anchored physical maps and molecular markers of chromosome groups 1 and 3 developed by WP1/2;
- chromosome landing by screening the MTP (Minimal Tiling Paths) pools with linked molecular markers and identification of a BAC contig from which additional markers will be designed and used to reduce the genetic interval around the target locus (Given this high resolution of markers, a single step should be sufficient to identify a BAC contig spanning the gene of interest);
- sequencing of the MTP at the target locus, annotation of the corresponding genomic sequence (WP5), and identification of candidate genes;
- validation of candidate genes by VIGS, allelic comparisons and/or stable transformation;
- identification of new alleles from core collections and gene bank materials as a method for isolating useful variants of the gene of interest.
For the first project year, WP3 partners have focused on establishing the genetic and molecular resources needed for fine genetic mapping of the targeted genes and QTL through (1) the construction of high-resolution mapping populations and (2) the collection of molecular markers in the target regions and the evaluation of their polymorphism in the parents of the mapping populations.
Deliverables:
D3.1: High resolution mapping of the 5 genes and QTL (May 2011)
D3.2: Identification of candidate genes on the MTP contigs (May 2011)
D3.3: Validation of candidate genes for YrH52, uniculm4 and S. nodorum resistance QTL by VIGS and/or allelic comparisons (May 2012)
D3.4: Validation of candidate genes by stable transformation (QTL, YrH52) (May 2012)
D3.5: Allelic diversity and expression analysis of candidate genes for uniculm4, grain yield and Stagonospora nodorum QTL (May 2012)
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Other WPs:
- WP1 - Establishment of genomic resources for physical mapping of wheat
- WP2 - Construction of anchored chromosome- and genomic physical maps of wheat and barley
- WP4 - Markers for molecular breeding
- WP5 - Bioinformatics
- WP6 - Dissemination, Technology Transfer and training
- WP7 - Management
This project is supported by the European Commission under the 7th Framework Programme
for Research and Technological Development